7/6/2023 0 Comments Xplorer xfangThis was expanded in its 1999 sequel, Star Ocean: The Second Story, which boasted as many as 86 different endings, with each of the possible permutations to these endings numbering in the hundreds, setting a benchmark for the number of outcomes possible for a video game. The original Star Ocean, published by Enix in 1996, introduced a "private actions" social system, where the protagonist's relationship points with the other characters are affected by the player's choices, which in turn affects the storyline, leading to branching paths and multiple different endings. The series is also known for being some of the earliest action RPGs to allow players to alter the storyline's outcome through the player's actions and dialogue choices, mainly through a social relationship system referred to as "private actions". Star Ocean is a franchise of action role-playing video games developed by the Japanese company tri-Ace and published by Square Enix (formerly Enix). The ToF-SIMS images of Cl − and PO 3 − were acquired at m/ z 35 and 79, respectively.The logo used for Star Ocean: The Last Hope (N) and (O) Merged ToF-SIMS images of Cl − (red) and PO 3 − (green) and the CLSM image of EYFP-HMGB1 (blue) or DAPI (blue). (L) and (M) ToF-SIMS images of: (L) Cl − and (M) PO 3 − using 3000 BIB scans. (J) Merged image of the ToF-SIMS images of the total ions, Cl − and PO 3 −. (G)–(I) ToF-SIMS images of: (G) total ions (H) Cl − and (I) PO 3 − using 100 BIB scans. (E) Merged image of the CLSM images of EYFP-HMGB1 (green), DAPI (blue) and ToF-SIMS image of the total ions (red). (D) ToF-SIMS image of the total ions using 10 BIB scans. (B) and (C) CLSM images of (B) EYFP-HMGB1 and (C) the DAPI stained nucleus. (A), (F) and (K) Bright field images of a single cell framed in a red box: (A) before (F) after 10 cycles of GCIB sputtering on (A) and (K) after 100 BIB scans on (F). COSIMSi of lyophilized HeLa cells without treatment using cisplatin. This journal is © The Royal Society of Chemistry.įig. This finding suggests that Smad3 and its related signalling pathway are most likely involved in the intracellular response to cisplatin induced DNA damage. More significantly, for the first time, similar integrated imaging revealed that the cisplatin lesions at Smad-binding elements, for example GGC(GC)/(CG) and AGAC, disrupted the interactions of Smad3 with DNA, which was evidenced by the remarkable reduction in the expression of Smad-specific luciferase reporters subjected to cisplatin treatment. The superposition of the fluorescence and the mass spectrometry (MS) signals indicate the formation of HMGB1-Pt-DNA ternary complexes in the cells. We utilized confocal microscopy imaging to map EYFP-fused HMGB1 and fluorescent dye-stained DNA in single cells, followed by the visualization of cisplatin using high spatial resolution (200-350 nm) time of flight secondary ion mass spectrometry (ToF-SIMS) imaging of the same cells. Herein, we developed a dual-modal microscopy imaging strategy to investigate, in situ, the formation of ternary binding complexes of the transcription cofactor HMGB1 and transcription factor Smad3 with cisplatin crosslinked DNA in single cells. However, the way in which cisplatin-induced DNA lesions regulate interactions between transcription factors/cofactors and genomic DNA remains unclear. 6 College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine Jinan 250355 People's Republic of China.Ĭisplatin is an extremely successful anticancer drug, and is commonly thought to target DNA.China 5 China Telecom Corporation Limited Beijing Research Institute Beijing 100035 People's Republic of China. 4 Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, Key Laboratory of Molecular Nanostructures and Nanotechnology, Institute of Chemistry, Chinese Academy of Sciences Beijing 100190 P.3 University of Chinese Academy of Sciences Beijing 100049 People's Republic of China.1 Beijing National Laboratory for Molecular Sciences, CAS Research/Education Center for Excellence in Molecular Sciences, National Centre for Mass Spectrometry in Beijing, CAS Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences Beijing 100190 People's Republic of China 2 Key Laboratory of Hubei Province for Coal Conversion and New Carbon Materials, School of Chemistry and Chemical Engineering, Wuhan University of Science and Technology Wuhan 430081 People's Republic of China.
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